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KMID : 0603519980030020115
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Journal of Korean Association of Cancer Prevention
1998 Volume.3 No. 2 p.115 ~ p.121
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A New Assay System for Analysing the Inhibitors of Tumor Invasion and Metastasis Using MMP-9/SEAP Vector
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Cha Hee-Jae
Jung Joo-Won
Lee You-Mie
Seiki Motoharu
Kim Ok-Hee
Kim Kyu-Won
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Abstract
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During the steps of tumor metastasis, matrix metalloproteinase-9 (MMP-9) is one of the most important enzymes and acts a key role to degrade the extracellular matrix. Therefore suppressing the expression of MMP-9 may inhibit the tumor invasion and metastasis. In this study, we developed the assay system to analyze the expression of MMP-9 by constructing the MMP-9 reporter vector and confirming the action of reporter protein by SEAP assay. Furthermore, we confirmed the expression pattern of MMP-9 is similar to that of Northern blot and gelatin zymogram assay. This assay system enables to detect the expression of MMP-9 by treating only simple methods. Therefore, we can apply this system to screen the MMP-9 inhibitors in various candidate compounds and factors.
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KEYWORD
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MMP-9, SEAP vector, Invasion, Metastasis
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